Prevention of H-Aggregates Formation in Cy5 Labeled Macromolecules
H-aggregates of the cyanine dye Cy5 are formed during covalent linkage to the cationic macromolecule Poly(allylamine) (PAH).The nonfluorescent H-aggregates strongly restrict the usage of the dye for analytical purposes and prevent a quantitative determination of the labeled macromolecules.The behavior of the H-aggregates has been studied by investi